Members of the genus Juglans are monecious wind-pollinated trees in the family Juglandaceae with highly heterozygous genomes, which greatly complicates genome sequence assembly. The genomes of interspecific hybrids are usually comprised of haploid genomes of parental species. We exploited this attribute of interspecific hybrids to avoid heterozygosity and sequenced an interspecific hybrid Juglans microcarpa × J. regia using a novel combination of single-molecule sequencing and optical genome mapping technologies.
Sequences ‘Jm’ represents J. microcarpa isolate 31.01, the maternal pseudohaplotype. Sequences ‘Jr’ represents J. regia cv. Serr, the paternal pseudohaplotype.
1. Browse J. regia Serr and J. microcarpa 31.01 pseudomolecules, gene annortation and transposable elements.
2. Download J.regia Serr and J. microcarpa 31.01 pseudomolecules and corresponding gene annotations.
3. BLAST online
Tingting Zhu, Le Wang, Frank M. You, Juan C. Rodriguez, Karin R. Deal, Limin Chen, Jie Li, Sandeep Chakraborty, Bipin Balan, Cai-Zhong Jiang, Patrick J. Brown, Charles A. Leslie, Mallikarjuna K. Aradhya, Abhaya M. Dandekar, Patrick E. McGuire, Daniel Kluepfel, Jan Dvorak, Ming-Cheng Luo. Sequencing a Juglans regia × J. microcarpa hybrid yields high-quality genome assemblies of parental species. Horticulture Research, 2019; 6 (1) DOI: 10.1038/s41438-019-0139-1
Walnut genome database 